TurnoverScout™

Proteins are subject to a constant process of degradation and resynthesis, and a comprehensive understanding of cellular protein turnover dynamics is critical to drug discovery research. TurnoverScout™ enables the independent and simultaneous tracking of protein synthesis and degradation rates for more than 8,000 proteins in parallel. This technology leverages a combination of SILAC metabolic labeling and TMT multiplexing, and can be combined with enrichment technologies for proteins to maximize sensitivity. The assay has been applied to more than 100 different cell lines and types, including iPSC-derived neurons and cardiomyocytes.

TurnoverScout™ provides deep insights into treatment-induced effects on cellular protein homeostasis, supporting a range of biopharmaceutical applications. The assay delivers critical information about drug safety and efficacy, particularly in the development and characterization of targeted protein degraders. Turnover analysis readily identifies the target(s) of these compounds, enabling lead optimization for selective degradation.